Technische Universität Wien
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Novel chemical imaging techniques for cancer research (PhD thesis)

Project synopsis

Mid-infrared (mid-IR) spectroscopy in the form of Fourier Transform IR (FTIR) spectrometers has long been established as a work horse for label free, non-destructive chemical characterization of bio materials. More recently, mid-IR hyperspectral imaging in combination with machine learning has been shown to be a powerful tool for medical diagnostics, especially for cancer diagnostics. The TUMOR-LN-oC project brings together a multinational multidisciplinary consortium ranging from medical professionals to laser physicists aiming combine super resolution IR imaging, organ on a chip technology and machine learning to gain new insights into cancer metastasis. The organ on a chip system will simulate the vascularized environment of around a tumor. IR spectroscopy will be used to collect images of individual, migrating cancerous cells with the aim of developing a rapid approach to their spectra classification based on comparatively old spectra-structure correlations and cutting edge machine learning approaches.

Scope of your contribution

You will be working in the Lendl group at TU Wien as part of the Horizon 2020 project “TUMOR-LN-oC”. Your thesis will encompass the implementation of new optical setups for mid-IR super resolution imaging, data acquisition and data evaluation. Within “TUMOR-LN-oC” you will network and interact with academic and industrial partners across Europe.

As with any doctoral thesis, here, a great deal of self-motivated, creative work is expected. The Lendl group has a positive, collaborative work environment. We are team of experienced graduate and post graduate students (physicists, chemists, chemical engineers, biomedical engineers) happy to lend a hand. Attendance of international scientific conferences and publishing of scientific articles is strongly encouraged.

Start date: March to May 2021.

Salary: 30 hours per week PhD position

 

Requirements

  • Masters in Physics, Chemistry, Electrical engineering, Biomedical Engineering
  • Interest in instrumental analysis, instrument development, creative problem solving
  • Prior experience in programming (Python, Matlab), electronics, optics, spectroscopy is advantageous but not required
  • Fluency in English is required.
  • Must be allowed to work in Austria.

How to apply

Please send your application to georg.ramer@tuwien.ac.at by February 13th 2021

You application should include an academic CV and a transcript of your Masters degree (If no Masters transcript is available yet, please provide your most recent academic transcript). Feel free to get in touch if you have questions about the application process.

The top applicants will be invited for a brief video interview in the third week of February.

Contact

Dr. Georg Ramer
georg.ramer@tuwien.ac.at

Prof. Bernhard Lendl
bernhard.lendl@tuwien.ac.at

Protein Refolding monitored by Laser-based IR Spectroscopy (Master Thesis)

Background and Motivation

IR spectroscopy is an excellent method for studying dynamics of protein secondary structure. However, its low sensitivity has been prohibitive for monitoring of protein refolding from inclusion bodies, which is an important step in downstream bioprocessing. Recent advancements in laser-based IR spectroscopy provided enhancement in robustness and sensitivity which are necessary for performing these measurements.

Scope of the work

You will be working in the Lendl at TU Wien together with a multi-disciplinary team of PhD students and PostDocs. This master thesis will be conducted within an FWF project dedicated to the advancement and application of laser-based IR spectroscopy for analysis of proteins in bioprocesses. Your work consists of IR measurements of proteins to study their secondary structure changes upon denaturation and protein refolding. Measurements will be performed with a novel laser-based IR transmission setup.
The thesis combines tasks in FTIR spectroscopy, liquid handling and optimizing experiment design. Students interested in extending their skills in these fields and in creative experimenting are encouraged to get in contact with us!

Requirements

     

  • Bachelor in Chemistry, Biomedical Engineering, or equivalent
  • Interest in instrumental analysis, spectroscopy, protein dynamics
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Further Information

Contact

Dr. Andreas Schwaighofer
andreas.schwaighofer@tuwien.ac.at

Prof. Bernhard Lendl
bernhard.lendl@tuwien.ac.at