Technische Universität Wien
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Preliminary Program

Tuesday, April 14th, 2020 

08:30

to

08:45

L

Registration and Welcome

08:45

to

09:00

L

Introduction of participants (e.g. name, work place, type of research, goal)

09:00

to

09:20

L

Theory: Fluorescence 2D gel electrophoresis and 2D Western Blotting (Sample Preparation, Sci-Dyes Labeling, 2D gel electrophoresis)

09:20

to

09:40

L

Image Analysis of 2D electrophoresis gel  - state of the art

09:40

to

10:00

L

Introduction to Modern Protein Mass Spectrometry

10:00

to

10:15

 

Coffee break

10:15

to

11:45

L

Sample Preparation (Sci-Dyes, Lightning Red), IPG strip rehydration and

Experimental setup of the workshop

11:45

to

13:00

 

Lunch

13:00

to

15:45

P

Gel analysis with demo gels under supervision (1)

15:45

to

16:00

 

Coffee break

16:00

to

17:00

L

Mass spectrometry

17:00

to

18:00

L/P

HPE Tower, HPE 2D Gels, Start 1st dimension: Cup Loading, start IEF

19:30 Dinner Invitation for all participants 

Wednesday, April 15th, 2020

08:00

to

09:00

P

Stop IEF, Equilibration of IPG, Start 2nd dimension (1)

09:00

to

10:30

P

2D gel electrophoresis (vertical) for Western Blotting

10:30

to

10:45

P

Coffee break

10:45

to

12:15

P

Gel analysis with demo gels under supervision  (2)

12:15

to

13:00

 

Lunch

13:00

to

14:00

Mass spectrometry - sample preparation

14:00

to

17:15

L

Mass spectrometry - analysis of mass spectra

17:15

to

18:00

L

Modern Mass Spectrometry for Proteomics

Thursday, April 16th, 2020

08:00

to

10:00

P

Scan gels

10:00

to

12:00

L

Gel analysis with demo gels under supervision  (3)

12:00

to

13:00

 

Lunch

13:00

to

14:30

L

Trouble shooting

14:30

to

14:45

P

Coffee break

14:45

to

17:00

P

MS

Friday, April 17th, 2020

08:00

to

10:00

L/P

Latest Developments in 2D electrophoresis, mass spectromety & software analysis

10:00

to

10:15

 

Break

10:15

to

11:45

L/P

Q &A


L: lecture, P: Hands-On

Small changes of time plan may occur please check regularly.