Fachbereich Instrumentelle Analytische Chemie / Bio- und Polymeranalytik
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Mass Spectrometry Imaging & Spray based Mass Spectrometry


Synapt HDMS G2

The Synapt HDMS is a high resolution mass spectrometer equipped with a nanoESI, a MALDI and a DESI source. Built as a Q-TOF, the device enables monoisotopic resolution over a mass range from small molecules to high molecular weight analytes (e.g. proteins) by accurate mass measurement. 

Furthermore the possibility of ion mobility separation leads to structural information of the analyte. Within this process ions are separated based on their shape (lipid vs. peptide with same m/z). 



The Ultraflex is a MALDI-TOF/TOF device, equipped with a 2000 Hz smartbeam laser, enabling high speed data  acquisition. The high mass resolution over a very broad mass range in combination with a high mass accuracy  facilitates very flexible analytical approaches. Furthermore laser diameter focuses down to 10 µm spatial resolution are possible, without loosing spectral quality and signal intensity.


Esquire Ion Trap

The Esquire 3000plus is an ion trap mass spectrometer (IT-MS) which is equipped with an orthogonal electrospray ion trap (ESI). If needed the iron source can be exchanged with an off-line nano electro spay, reducing the amount of sample needed to 1-2 µL.

Adjustment of the needle is done with the help of a built-in camera which allows detailed observation of the spay process. The mass range of the analyzer, the ion trap, is 3 000 m/z. It delivers well resolved spectra throughout the entire mass range.


HCT ion trap

The HTCplus is also an ion trap mass spectrometer (IT-MS) which is by default equipped with an orthogonal electrospray ion trap (ESI). Compared to the Esquire 3000plus this instrument is characterized with a better sensitivity, a greater dynamic range and a faster scan speed.

In our lab this instrument is operated with a pulsed dynamic focusing (PDF)-MALDI ion source. This ion source works under atmospheric pressure and is, therefore, a useful supplement to high-vacuum MALDI.



Molecular weights of biopolymers are nowadays mostly determined by mass spectrometry. This however requires bringing them into high-vacuum which might bias the molecular weight measurement.

Gas-Phase Electrophoretic Molecular Mobility Analysis (GEMMA) is an alternative way of molecular weight detection. Electrophoretic mobilities are measured at ambient pressure with an electrospray/differential mobility analyzer. By comparing the electrophoretic mobility diameters with those of well-defined macro molecules, molecular weight determination is possible.