Technische Universität Wien
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Time-Resolved FTIR-Spectroscopy for Analysis of Refolding Kinetics of Proteins (Master Thesis)

Background and Motivation

FTIR spectroscopy is an excellent method for studying protein secondary structure. By applying dedicated experimental techniques such as rapid-scan or step-scan spectroscopy, time-resolved spectra acquisition can be obtained with millisecond and nanosecond time resolution. For time-resolved spectroscopy of non-reproducible experiments, the rapid scan technique is employed. Here, this time- resolved technique will be applied to study protein structure change of a model protein after temperature jump excitation for analysis of the refolding kinetics in the millisecond time range.

Scope of the work

The student will be working in the Lendl lab at TU Wien together with a multi-disciplinary team of Master and PhD students as well as PostDocs. The work consists of setting up a temperature-jump experiment for studying conformational changes in a protein model system after thermal perturbation by rapid scan FTIR spectroscopy.
The thesis combines tasks in FTIR spectroscopy, liquid handling and optimizing experiment design. Students interested in extending their skills in these fields and in creative experimenting are encouraged to get in contact with us!

Requirements

  • Bachelor studies in Physics, Chemistry or equivalent

  • Interest in instrumental analysis, spectroscopy, protein dynamics

Contact

Dr. Andreas Schwaighofer
andreas.schwaighofer@tuwien.ac.at
Prof. Bernhard Lendl
bernhard.lendl@tuwien.ac.at